This Reference Guide and Fee Schedule for the services of the MDA Animal Health Diagnostic Laboratories goes into effect on March 1, 2006. Please click on the items in the table of contents below to be directed to that item.
Lab Fee Listing
Laboratory Submission Forms for non-regulatory testing:
Contact the laboratory directly for special or regulatory test forms.
General Information: Submit complete information with all samples. Include:
Tissue & Body Fluid: Tissues should be collected with the least possible contamination. Select samples of organ tissues before incising the gastrointestinal tract. Tie off 3" intestine sections for submission. Individual organs should be placed in separate containers i.e. whirlpak bags or wide-mouth, screw cap specimen containers. Tissues for bacteriology should be chilled in transit by icepacks. Formalized tissues are not acceptable for microbiological culture. For viral isolation, freeze tissue, pack in airtight container (CO2 is VIRICIDAL) and transport on dry ice.
Serum: Bring samples to the lab no later than 4 PM on the day blood is collected, unless you have separated the serum and refrigerated it. If more than one test is requested, please submit 1 ml of serum for each additional test. Blood samples held too long prior to submission may be unsuitable for testing.
Brucellosis Tests: Bangs tubes may be picked up at any of the Animal Health Laboratories. It is best to submit samples early in the week. Try to get at least 5 ml of blood from each animal; obtaining 10 ml is preferable. Keep samples cool; do not freeze unless you have separated the serum from the clot. Usually this is done by personnel at the laboratory. Brucellosis must be submitted with the appropriate form.
Pseudorabies and Other Tests Done in Tissue Culture: Samples should be submitted in sterile vacuum tubes or clear plastic screw cap tubes with no additives. Bring blood to the laboratory immediately. Swine blood in particular hemolyzes rapidly and hemolyzed serum is unsuitable for testing. Submit samples early in the day as we must transfer the serum to other tubes before the red cells hemolyze. Keep samples cool. Use ice packs if needed.
AGID Test for Equine Infectious Anemia (EIA) & Bovine Leukemia Virus (BLV): Silicone coated vacuum tubes are preferred. EIA tests must be submitted with the appropriate state or federal form.
Anaerobic culture: Material from the site of the infection may be aspirated by syringe, capped, refrigerated, and transported as soon as possible. Tissue should be 2" cubes refrigerated and transported within 4 hours to the laboratory.
Blood Culture: Blood samples must be collected without introducing contaminants from the skin. The blood must be inoculated immediately into a sterile blood culture bottle. Blood culture bottles which require collection by syringe and then inoculation into the bottle may be used. Please Note: Vacuum tubes designed for hematology or serology are usually not sterile and are not suitable when collecting blood for microbiological examination (Blood culture bottles are designed to neutralize the inhibitory effects of serum immediately and allow the microbes to grow).
Fungal Culture: Hair/skin scrapings are collected by sterile forceps or scalpel and submitted in clean paper packets or envelopes. (Specimens in closed tubes retain moisture and contaminants overgrow the material.) Skin should be cleansed with alcohol before scraping. Do not refrigerate while transporting. Do not use transport swabs and media designed for bacterial isolation.
Hematology: EDTA blood tubes for blood counts. Samples should be submitted to the lab as soon as possible. Blood samples held too long may not be suitable for testing.
Two thin blood smears on frosted slides for differential counts should be made prior to adding blood to anticoagulant or no later than 15 minutes after adding blood to EDTA tube and submitted with sample. Slides should have frosted end and be labeled in pencil.
CEM Culture: Performed at the Frederick Animal Health Laboratory only. For CEM cultures, please make prior arrangements with the laboratory (301) 600-1548. Strict adherence to federal regulations is required.
Feces: Submit from 1/2 to 2 oz. of feces in a sterile, 4 oz. screw-cap container. For bacterial and viral examination, use no preservatives. Cryptosporidium requires 3-5 grams of feces in 15 ml of 10% formalin. Transport specimen under the following conditions:
(Note: For Campylobacter isolation, specimen must be transported to the lab within 2 - 4 hours max, even when refrigerated).
Milk: Collect milk aseptically as soon as possible after mastitis is noticed and before antibiotic treatment begins. Teats must be clean and thoroughly dried.
Points to Remember:
Routine aerobic culture (up to 3 isolates) per tissue or swab; antibiotic sensitivity will be run at no extra cost if indicated.
Special Cultures (Listeria, anaerobic, Campylobacter, Salmonella, Candida, etc.) charges per bacterial species requested, per tissue or swab.
Bacterial culture identification (bacteriologic plates submitted with growth, for identification). Fee is per plate (up to 3 isolates). Animal species of origin must be indicated at the time of submission.
Antibiotic sensitivity if submitted separately as a bacterial plate fee is assessed per isolate.
Federal regulations govern the packaging and labeling of diagnostic specimens. If spillage occurs during transit that damages other mail, equipment or causes harm to personnel, the shipper may face prosecution even if the material involved was not infectious or hazardous.
The box containing samples should be labeled on the outside as "Animal Clinical (Diagnostic) Specimen". Specimens should be "triple bagged". The primary container should be a watertight primary receptacle (Whirl Pak and Zip Locks frequently leak if not closed properly) followed by a watertight secondary packaging. An absorbent packing material must be placed between the primary receptacle and secondary packaging. Screw-cap closures must be reinforced with tape. The final packaging should consist of sturdy construction (corrugated fiberboard, wood, metal or plastic).
Packaging Instructions- The following is provided to prevent problems associated with improper packing.
Fees for services on specimens submitted by individuals without an established account must be paid at the time of submission. Presently we only accept cash and checks made payable to the Maryland Department of Agriculture (we cannot accept credit card payments). Accounts 75 days past due will be assessed a 17 percent collection fee.
Test results and reports are confidential and are available only to the veterinarian, clinic or other authorized submitter of the samples. Owner identity may be difficult to establish unless clearly listed on admission form.
Referrals: The Animal Health laboratories will automatically refer any sample for tests requested but not performed by our laboratories to another laboratories for testing. The destination will vary according to factors such as availability, reliability, cost and turn-around time for results. There will be a shipping fee and a small handling charge (no shipping fee for samples sent to Antech).
Avian Serum: For avian serum to separate well, the tubes should be filled about 1/4 full of blood and immediately placed in a slanted position until the clot is set. Lay the tube on its side with the top edge propped up just enough to keep the blood from running out. Get as much surface area as possible. Leave undisturbed for about 30 minutes. The tube may then be placed in an upright position and serum will continue to separate. Avian serum separates best when kept relatively warm. Wait a few hours before refrigerating unless the day is extremely hot. Never freeze any serum which is to be tested for mycoplasma antibodies.
We can test for Pullorum-typhoid, Mycoplasma gallisepticum, Mycoplasma synoviae, and Avian Influenza on serum.
Avian Tissues/Swabs: Tissues and culturette swabs (fresh/frozen) can be tested for common poultry viruses and bacteria at the Salisbury Laboratory . They should be transported overnight in properly chilled conditions. Call the Salisbury lab before shipping samples. Phone: (410) 543-6610
Training of NPIP Testing Agents: The Animal Health Program offers training of testing agents to perform blood testing of poultry to qualify flocks as National Poultry Improvement Plan participants or for exhibition. For information call the Salisbury Lab, (410) 543-6610
Testing of Poultry for Pullorum/Fowl Typhoid, and certain other diseases is offered for a fee (see fee schedule) in areas or situations when private testing agents are unavailable. Avian Influenza testing for shows, interstate movement, and NPIP certification is offered for free. Call the Field Veterinarian or Livestock Inspector at the MDA lab nearest to you.
Poultry Health Consultation is available from the Salisbury Laboratory, (410) 543-6610. Owners submit birds for necropsy to the nearest MDA lab. It is recommended that owners call the lab first prior to submitting samples.
Clinical Pathology/Hematology We offer complete Clinical Chemistry and Hematology analysis through all the MDA Laboratory system. Some tests are performed at the Animal Health Laboratories; some are referred to other laboratories. There is no shipping charge for referrals to Antech and most routine test results are faxed to us the next day.
Chemistry Profiles available through Antech: Superchem: Albumin, Alk Phos, ALT, Amylase, AST, BUN, Calcium, Chloride, Cholesterol, CPK, Creatinine, GGT, Globulin, Glucose, Lipase, Magnesium, Osmolality, Phosphorus, Potassium, Sodium, Total Bilirubin, Total Protein, Triglyceride. Vet Screen: Albumin, Alk Phos, ALT, BUN, Calcium, Chloride, Cholesterol, CPK, Creatinine, Globulin, Glucose, Phosphorus, Potassium, Sodium, Total Bilirubin, Total Protein. Pre Op Screen: albumin, Alk Phos, ALT, BUN, Creatinine, Globulin Glucose, Total Protein Mini Screen: ALT, BUN, Glucose, Total Protein. Total Body Function: Superchem CBC, T4 Canine Autoimmune Profile: CBC, Coombs, RA, ANA, Platelet Count Coagulation Profile 1: CBC, Platelet Count, PT, PTT, Fibrinogen, D-Dimer, Plasma Protein Renal Profile: Albumin, BUN, Calcium, Creatinine, Globulin, Phosphorus, Potassium, Sodium Total Protein, CBC, Urinalysis Liver Profile: Alk Phos. ALT, AST, BUN, GGT, Globulin, Glucose, Total Bilirubin, Total Protein, CBC, Pr and Post Bile Acids.. Electrolyte Profile: Sodium, Potassium, Chloride Equine Training Profile: SGOT, CPK, CBC Molecular Genetics: PCR samples should be submitted well refrigerated, but not frozen.
Equine Neurologic Syndrome: horses exhibiting central neurologic signs in the absence of noninfectious cause, or where infectious cause is suspected and cannot be ruled out. Differentials include: Rabies, EHV, WNV, EPM, EEE. Call state lab, collect nasal swab, red top and purple top blood tubes.
Urinalysis: Specimens should be submitted in sterile, leak proof containers. Example: screw-cap cup or tube. Catherization is the preferred method of collection, 10-20 ml per sample. If free catch void urine is submitted, discard initial 15-30 ml to reduce contamination.
For analysis of fecal samples, please submit fresh, chilled feces (unless otherwise noted) to decrease deterioration and bacterial overgrowth. Samples held for more than 2 day between collection and submission to the lab may be unsuitable for testing.
All toxicological tests are sent to reference laboratories, which involves additional charges. Please check with the lab director or pathologist regarding charges for testing and shipping. Purity of sample is of extreme importance in toxicology.
Certification programs are administered by MDA's Animal Health Program in cooperation with the U.S. Department of Agriculture but rely on testing and inspection of animals by private veterinary practitioners who are accredited by USDA/MDA to perform official duties. Owners should contact their veterinarian for details.
Cattle and Goat Herds can be officially accredited tuberculosis free and/or certified Brucellosis free Swine Breeding Herds can become validated Brucellosis free and/or pseudorabies qualified negative; Feeder pig Herds can become pseudorabies monitored. Sheep Flocks must participate in the scrapie flock certification program.
Poultry Flocks and Hatcheries can qualify for a certification in several programs of the National Poultry Improvement Plan (NPIP). (Contact Salisbury Lab for information)
Livestock to be shown in Maryland or Moved Interstate from Maryland for showing or other purposes must be accompanied by a Certificate of Veterinary Inspection issued by an accredited private veterinarian and endorsed by MDA at its Annapolis Office, (410) 841-5810, or Frederick Animal Health Lab, (301) 600-1548.
Import-Export Information (international) is not available from MDA but rather from USDA/Aphis/Veterinary Services in Annapolis, (410) 571-0330. For information on quarantine in Maryland for equine breeding stock imported from certain countries, call MDA's Animal Health Office in Annapolis, (410) 841-5810.
For animals originating in Maryland, a valid intrastate Maryland CVI Show Form (form MD-E-16) must be filled out and completed by an accredited veterinarian within 60 days of the first exhibition. The date of the first show or fair and all other Maryland exhibitions you expect to attend should be recorded on the CVI. Each animal must be individually and positively identified on the health chart. The CVI that is to be used at a subsequent fair or show will be evaluated, initialed and dated by an MDA Animal Health official at each event.
All animals entering Maryland must satisfy import health and test requirements for that species and be listed on an interstate CVI with each animal individually and positively identified on the health chart. The interstate CVI is valid for 30 days from the date of issuance with regard to entrance into Maryland. The interstate CVI must include the names and dates of MD shows you are expecting to attend. A poultry Premise Registration Number will be required for Maryland poultry producers to exhibit at 2006 fairs and shows.
In consideration of public health concerns, it is strongly recommended that all mammals to be exhibited at any fair or show in Maryland be vaccinated against rabies if there is an approved vaccine for the species. This is especially true for animals that will be in petting zoos.
Johne's Disease is a chronic (years) mycobacterial infection that affects principally the lower small intestine of many ruminants. Cattle, sheep, goat and less frequently deer, llamas, bison and other ruminants are all susceptible to the disease. The clinical condition is caused by Mycobacterium avium paratuberculosis infection.
The economic impact of Johne's Disease in a herd may include some or all of the following effects: premature culling of exposed, infected or clinical animals which may lead to loss of valuable animals and genetic potential; decreased milk production in infected animals (losses of 5-20%); loss of marketing and show opportunities; possible breeding problems in infected animals; veterinary costs.
In the living animal, fecal culture is the most accurate diagnostic test, but requires 12 to 16 weeks culture time and is expensive. Fecal culture sensitivity is considered to be about 40% +/-10% and its specificity is 99% if done correctly. Fecal culture tests cannot detect Stage I or some Stage II animals. Various serologic tests including ELISA and AGID tests detect antibody in the serum and can be used on a herd basis or to confirm clinical cases. The serology tests are less accurate than culture, but are relatively rapid and less expensive. Only in advanced clinical cases are the predictive values of AGID tests similar to culture and ELISA values. ELISA has been most widely used for screening herds. ELISA sensitivity for clinical cases has been reported to be 85%, while the sensitivity is about 15% in non-clinical cases. In the dead animal, Johne's disease is accurately diagnosed by histopathology of the lower small intestine (distal ileum) and associated lymph nodes.
In herds with low to moderate infection (one percent or fewer clinical cases per year), wise use of a combination of testing, culling and management can be expected to reduce the number of clinical cases to zero within one to three years and to eliminate most infection in five to seven years. As the herd turns over, each succeeding generation will have fewer infected animals, all of which will eventually be non-shedders. Complete elimination of infected cattle is likely to take many years after Johne's disease becomes invisible in the herd. Biosecurity practices should remain in place, otherwise Johne's disease is likely to recur.
With a negative herd test, Maryland cattle herds can qualify for the U.S. Johne's Disease Herd Status Program for Cattle. This program was adopted by the United States Animal Health Association in 1998. For more information on control programs, contact:
Maryland Johne's Disease Coordinator Maryland Department of Agriculture Frederick Animal Health Laboratory 1840 Rosemont Avenue Frederick, MD 21702 301-600-1548
More information can be found on the USDA Animal and Plant Health Inspection Service (APHIS) website: www.aphis.usda.gov/vs
To report a fish kill, please call the Maryland Emergency Management Agency at: Phone: 1-877-224-7229
For Fish Health Investigation information, please call the Maryland Department of Natural Resources at: Phone: 1-800-628-9944
For Aquatic Animal Permitting Issues, including movement in or out of state, please contact Rich Bohn, MD DNR Fisheries Service at: 410-260-8317 or email email@example.com
Full necropsy service, including gross examination of animal carcasses, is available in our lab system. Veterinary diagnosticians and pathologists will perform the gross examination, and collect specimens for additional laboratory tests. The necropsy and disposal fee includes gross and histopathologic examination, most bacterial and viral identification procedures, parasite identification and cremation. Toxicology tests are not included in the necropsy and disposal fee; samples for toxicology are sent to reference labs. Additional fees will be incurred for toxicology tests.
Carcasses intended for necropsy should be kept refrigerated, but not frozen, as freeze/thaw artifacts obscure gross and microscopic lesions. Because of public health concerns, we are not able to return carcasses or parts. We do not perform cosmetic necropsies. Please contact our labs for alternate sites that provide disposal only services.
Necropsies of food animals*, horses and backyard poultry are done at the Frederick laboratory. Commercial and backyard poultry necropsies are done at Salisbury laboratory; necropsy for other species is limited to specific cases as determined by the laboratory director or State veterinarian.
Biopsy service: Tissue should be placed in 10% neutral buffered formalin. Ten volumes of formalin are required to adequately fix one volume of tissue. Specimen should consist of the lesion site and surrounding tissue. Do not allow fresh or fixed tissue to freeze.
No charge if only head is submitted for domestic/farm animals. (DO NOT REMOVE HEAD FROM WILD ANIMALS – submit entire carcass). If entire animal is submitted, the disposal fee is based on weight, except for wild animals. If rabies exam is performed as part of a necropsy, the necropsy fee will apply, with no extra charge for rabies testing.
Every rabies sample must be identified with the following information:
The fees listed in this schedule are effective March 1, 2006 and were set by the Maryland Department of Agriculture. They are subject to change without notice.
The Maryland Department of Agriculture laboratories reserve the right to send samples to outside labs if necessary. Shipping and handling fees will be charged.
1840 Rosemont Ave Frederick, MD 21702Phone: 301-600-1548Fax: 301-600-6111AHFrederick.firstname.lastname@example.org
Above: Photo of the Salisbury Animal Health Lab Staff
27722 Nanticoke Rd. Salisbury, MD 21801Phone: 410-543-6610Fax: 410-543-6676AHSalisbury.email@example.com
50 Harry S. Truman Parkway, Annapolis, MD 21401